Table 1.
Statistical analysis of hippocampal CA1 neurons reconstructed from RyR2fl/fl mice transfected with rAAV.CamK2α.GFP (WT) vs rAAV.CamK2α.cre-GFP (KO).
| Neurons | WT | KO | p value | Sum |
|---|---|---|---|---|
| Basal dendrites | ||||
| Length (μm) | 2268 ± 185.5 | 1381 ± 149.2 | 0.0006 | *** |
| Surface area (μm2) | 14672 ± 1223 | 8700 ± 905.1 | 0.0003 | *** |
| Volume (μm3) | 8391 ± 743.5 | 4859 ± 554.1 | 0.0004 | *** |
| Dendritic thickness (μm) | 2.193 ± 0.04045 | 2.04 ± 0.04509 | 0.0173 | * |
| Number branch points | 35.44 ± 3.58 | 21.14 ± 2.925 | 0.0035 | ** |
| Apical dendrites | ||||
| Length (μm) | 2938 ± 271.3 | 2386 ± 161.5 | 0.0729 | ns |
| Surface area (μm2) | 20193 ± 1724 | 15494 ± 1205 | 0.0277 | * |
| Volume (μm3) | 12042 ± 992.9 | 8749 ± 779.9 | 0.0128 | * |
| Dendritic thickness (μm) | 2.318 ± 0.05193 | 2.159 ± 0.0321 | 0.0100 | * |
| Number branch points | 34.5 ± 4.863 | 32.1 ± 3.8 | 0.6964 | ns |
| Total | ||||
| Length (μm) | 4810 ± 461.6 | 3335 ± 282.5 | 0.0100 | * |
| Surface area (μm2) | 32122 ± 2921 | 22280 ± 2032 | 0.0097 | ** |
| Volume (μm3) | 18767 ± 1674 | 12936 ± 1403 | 0.0133 | * |
| Dendritic thickness (μm) | 2.264 ± 0.05678 | 2.08 ± 0.04186 | 0.0197 | * |
| Number branch points | 65.55 ± 9.009 | 44.54 ± 5.7 | 0.0543 | ns |
| Spines | WT | KO | P value | Sum |
|---|---|---|---|---|
| S. oriens | ||||
| Density | 9.556 ± 0.7015 | 6.004 ± 0.842 | 0.0037 | ** |
| % Mushroom | 59.47 ± 1.652 | 46.64 ± 2.726 | 0.0005 | *** |
| % Thin | 10.18 ± 0.9954 | 8.701 ± 1.229 | 0.3569 | ns |
| % Stubby | 30.25 ± 1.579 | 44.45 ± 3.074 | 0.0003 | *** |
| Spine length (μm) | 1.217 ± 0.0193 | 1.095 ± 0.0254 | 0.0001 | *** |
| S. radiatum | ||||
| Density | 11.4 ± 0.6692 | 7.797 ± 1.195 | 0.0137 | * |
| % Mushroom | 56.86 ± 2.124 | 53.98 ± 2.891 | 0.4356 | ns |
| % Thin | 12.01 ± 1.481 | 12.8 ± 1.381 | 0.7026 | ns |
| % Stubby | 31.13 ± 1.428 | 33.22 ± 2.051 | 0.4170 | ns |
| Spine length (μm) | 1.224 ± 0.02199 | 1.219 ± 0.0227 | 0.8663 | ns |
| S. lacunosum moleculare | ||||
| Density | 8.444 ± 0.6342 | 4.529 ± 0.34 | <0.0001 | **** |
| % Mushroom | 55.99 ± 2.851 | 46.88 ± 3.025 | 0.0386 | * |
| % Thin | 18.14 ± 1.475 | 15.45 ± 1.878 | 0.2639 | ns |
| % Stubby | 25.87 ± 2.218 | 37.32 ± 3.003 | 0.0046 | ** |
| Spine length (μm) | 1.396 ± 0.02258 | 1.29 ± 0.03129 | 0.0068 | ** |
| Total | ||||
| Density | 9.602 ± 0.425 | 5.848 ± 0.4809 | <0.0001 | **** |
| % Mushroom | 57.32 ± 1.865 | 47.96 ± 1.674 | 0.0022 | ** |
| % Thin | 12.84 ± 1.126 | 12.88 ± 1.1 | 0.9771 | ns |
| % Stubby | 29.79 ± 1.268 | 38.9 ± 1.568 | 0.0005 | *** |