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. 2020 Jul 24;28(1):170–183. doi: 10.1038/s41418-020-0591-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic diagram showing different domains of PDCD11 consisting of Ribosome (Rib, 1-1407 aa), Coil (1408-1871 aa), and Terminal (Ter, 1742-1871 aa) domains. b Rescuing effects on apoeb positive microglia of pdcd11 mutants with the Rib or Coil domain mRNA overexpression. Red arrows indicate the apoeb positive cells. Scale bar: 100 μm. c qPCR assessment of inflammatory genes expression in pdcd11 mutants with Rib or Coil domain of PDCD11 overexpression. d Western blot examination of the 536-serine modified P65 expression in 22 hpf WT and pdcd11 mutants. e Western blot examination of nuclear P65⁵³⁶ contents with the Coil or Ter overexpression in HEK293T cells. f Immunofluorescence assay showing the expression pattern of cells co-transfected with N-terminal Flag fused Coil and P65-GFP. White arrowheads indicate the colocalized Coil and P65 in the cytoplasm or nucleolus. Scale bar: 20 μm. g Representative image of three independent experiments showing the effect of increasing Coil transfection on nuclear/cytoplasm P65 content. h Representative image of three independent experiments showing P65⁵³⁶ levels in WT and pdcd11 mutants with Coil domain (Coil), Terminal domain (Ter), or Ribosome domain (Rib) mRNA overexpression. i Appearance of mfap4 expressing macrophages in pdcd11 mutants treated with NF-κB inhibitor. Scale bar: 50 μm. j Rescuing effect of NF-κB inhibitors on apoeb positive microglia in pdcd11 mutants. Scale bar: 100 μm. k Representative image of three independent experiments showing the rescue effects of Tgfβ1 levels with Coil domain (Coil), Terminal domain (Ter), Ribosome domain (Rib) mRNA or NF-κB inhibitor addition in 22 hpf pdcd11 mutants. The number positioned in the upper right corner of b and lower right corner of i, j represent the number of zebrafish embryos shown positive phenotypes versus the total number of embryos examined. Means ± SEM are shown for three independent experiments. n.s not significant, ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 (Student’s t test).