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. 2020 Aug 19;28(1):283–302. doi: 10.1038/s41418-020-0600-6

Fig. 6. Fluorochrome-labeling and histological analysis of in vivo bone regeneration.

Fig. 6

a The area of Tetracycline-labeled, Alizarin Red-labeled and Calcein-labeled bone was elevated by Lenti-circRNA-vgll3 group, whereas the circRNA-vgll3 inhibitor reduced this area. Scale bars: 1000 µm. b van Gieson’s picrofuchsin staining showed that Lenti-circRNA-vgll3 increased the new bone percentage in the scaffold tissue whereas the circRNA-vgll3 inhibitor impaired it. Scale bars: 1000 µm. c Immunohistochemical analysis of Itga5, Runx2, OSX and OPN in the defect area at 8 weeks post-operation. Scale bars: 25 µm. *P < 0.05, statistical analysis was performed by one-way ANOVA.