Table 2.
Primers used for quantitative RT-PCR.
| Genes | Forward (5′-3′) | Reverse (5-3′) | Annealing temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|
| Col1a1 | GACTGTCCCAACCCCCAAAA | CTTGGGTCCCTCGACTCCTA | 60 | 102 |
| Runx2 | ATCATTCAGTGACACCACCA | GTAGGGGCTAAAGGCAAA AG | 60 | 141 |
| OSX | GGA AAA GGAGGCACA AAGAA | CAGGGG AGA GGAGTCCAT T | 60 | 106 |
| OPN | CCAGCCAAGGACCAACTACA | AGTGTTTGCTGTAATGCGCC | 60 | 132 |
| OCN | CAGACA AGTCCCACACAGCA | CCAGCAGAGTGAGCAGAG AG | 60 | 76 |
| Itga5 | CAG ATAGCA CCCGAGTTACCA | ACAATCCTACCTGCCCTA ACG | 60 | 113 |
| Vgll3 | CCAACACCTTGCATCCCGAA | GGTCTGCTGTTGTAAAGGTGC | 60 | 213 |
| AdipoQ | CCACCCAAGGAAACTTGTGC | GACCAAGAACACCTGCGTCT | 60 | 136 |
| SOX9 | GTCGGTGAAGAATGGGCAAG | GACCCTGAGATTGCCCGGA | 60 | 161 |
| GAPDH | AAGAAACCCTGGACCACCCAGC | TGGTATTCGAGAGAAGGGAGGG | 60 | 178 |
| CircRNA-vgll3 | GGAGCCTTTCATGGGACAGT | CTGCTGTTGTAAAGGTGCCGTG | 60 | 196 |