Fig. 6. SEPN1 regulates ER-mitochondria Ca²⁺ homeostasis.

a WT and SEPN1 KO HeLa cells were co-transfected with ER aequorin-based Ca²⁺ sensors and SEPN1 plasmids and calcium refilling of the ER was recorded (Ca²⁺ 1 mM) (N = 10). The traces are representative of ten independent experiments that led to similar results. b Measurements of [Ca²⁺] using recombinant aequorin-based Ca²⁺ sensors in the mitochondria upon agonist stimulation (100 μM Histamine) (N = 10). The traces are representative of ten independent experiments that led to similar results. c Flag and beta-Actin representative immunoblot of proteins extracted from SEPN1 KO HeLa cells transfected with SEPN1 wt-Flag, SEPN1 CC-Flag, and SEPN1 SS-Flag.