Fig. 5.

CD4⁻CD8αβ⁺ T cells suppress T helper function and gain enhanced cytotoxic function. a–c Total lymph node cells from LLO WT or LLO Vps34^KO mice were stimulated in vitro with LLO190-205 for 120 h. Surface expression of CD40L (a) and Ox40 (b) and intracellular expression of granzyme B/IFN-g (c) was analyzed in TCR Vα2⁺ CD4⁻ and TCR Vα2⁺ CD4⁺ cells. d Splenic lymphocytes from C57BL/6 mice infected with LCMV Armstrong for 7–12 days were harvested and analyzed for intracellular granzyme B/IFN-g levels among LCMVgp66-77-I-A^b+ CD44^hi CD4⁻ and CD4⁺ T cells. n = 2 from 2 independent experiments for (a) and (b), n = 5 from 2 independent experiments for (c) and n = 12 from 3 independent experiments for (d)