Fig. 6.

Effector CD4⁻ MHCII-recognizing T cells shift towards CD8⁺ lineage transcriptional programming. Total lymph node cells from LLO WT and LLO Vps34^KO mice were stimulated with LLO190-205 in vitro for 120 h. Effector CD4⁻ T cells (TCR Vα2⁺ CD4⁻) and conventional effector CD4⁺ T cells (TCR Vα2⁺ CD4⁺) were FACS-sorted and expression of 16T cell lineage-specific transcripts was analyzed by qPCR. a Heatmap representation of Pearson product-moment correlation coefficients for the 4 sample groups. b mRNA expression levels for each target are presented relative to expression levels in LLO WT effector CD4⁺ T cells. Targets are grouped based on function (Lineage identity, Effector molecules or Master Txn factors). Expression levels in in vitro-stimulated pMel-1 CD8⁺ T cells were also assessed for a CD8⁺ lineage reference. Primer sequences are listed in Table S2. n = 9 from 4 independent experiments. Y-axis label shown for Lineage Identity also applies to Effector Molecules and Master Txn Factors. Statistical significance was determined by the Mann–Whitney U Test; *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not significant