Fig. 4.

Bcl10 is required for the suppressive function of Treg cells. CD45.1⁺CD4⁺CD25^-CD44^-CD62L⁺ naive T cells were isolated from the spleen and lymph nodes of CD45.1⁺ mice, and CD45.2⁺CD4⁺YFP⁺ Treg cells were isolated from Bcl10^+/+Foxp3^cre (WT) or Bcl10^fl/flFoxp3^cre (KO) mice. Naive T cells alone (Naive T + PBS) or with WT Treg (Naive T + WT Treg) or KO Treg (Naive T + KO Treg) cells were intravenously injected into Rag1^−/− recipient mice. a Body weight changes in Rag1^−/− recipient mice receiving different combinations of T cells in the T cell transfer-induced colitis model. b H & E staining of the small intestine of Rag1^−/− recipient mice at 18 weeks after receiving different combinations of T cells. c Absolute numbers of IFNγ- and IL17A-producing CD45.1⁺ T cells in the lamina propria of the large intestine (LPL) after ex vivo stimulation with PMA (50 ng/mL) and ionomycin (500 ng/mL) for 4 h. d Absolute numbers of IFNγ- and IL17A-producing CD45.1⁺ T cells in the spleen after ex vivo stimulation with PMA (50 ng/mL) and ionomycin (500 ng/mL) for 4 h. e and f In vitro Treg-cell suppression assay: CFSE-labeled naive CD4⁺ T cells (from CD45.1⁺ mice) cultured alone or cocultured with WT (from Bcl10^+/+Foxp3^cre mice) or KO (from Bcl10^fl/flFoxp3^cre mice) Treg cells at a 1:1 e or 2:1 f ratio in the presence of irradiated splenocytes and an anti-CD3 antibody. Student’s t test was used as the statistical test (*p < 0.05, **p < 0.01, and ***p < 0.005). All error bars represent SDs