Fig. 5.

Bcl10 is essential for maintaining the identity and population of Treg cells. Spleens and peripheral lymph nodes from 24-day-old Bcl10^+/+Foxp3^cre or Bcl10^fl/flFoxp3^cre mice were analyzed by FACS (n = 5, 4, or 3 per group). a FACS plots represent CD4⁺CD25⁺Foxp3⁺ (Treg) cells gated on the CD4⁺CD8⁻ cells in the spleen or peripheral lymph nodes (above). Statistical analyses were used to evaluate the CD4⁺CD25⁺Foxp3⁺ cell percentage in the CD4⁺CD8⁻ cell population and the absolute numbers in the spleen or peripheral lymph nodes (below). b FACS plots represent effector (CD44^hiCD62L^lo) and naive or central (CD44^loCD62L^hi) cells gated on CD4⁺YFP⁻(Foxp3⁻) (above) or CD4⁺YFP⁺(Foxp3⁺) (below) cells in the spleen and lymph nodes (above). Statistical analyses were used to evaluate the effector (CD44^hiCD62L^lo) and naive or central (CD44^loCD62L^hi) cell percentages in the CD4⁺YFP^-(Foxp3^-) (above) and CD4⁺YFP⁺(Foxp3⁺) (below) cell populations in the spleen and lymph nodes. c WT (from Bcl10^+/+Foxp3^cre mice) and Bcl10 KO (from Bcl10^fl/flFoxp^cre mice) Treg cells were stimulated with PMA (20 ng/mL) and ionomycin (200 ng/mL) for the indicated time, followed by immunoblotting analysis. d FACS plots represent KLRG⁺ cells gated on the CD4⁺YFP⁺ cells in the spleen and lymph nodes. e The percentage of KLRG⁺ cells gated on the CD4⁺YFP⁺ cells in the spleen and lymph nodes (WT: Bcl10^+/+Foxp3^cre mice (n = 4), KO: Bcl10^fl/flFoxp3cre mice (n = 3)) is shown. f and g The mRNA levels of Klrg1, Icos f, Tgfb1, Il-10, Rel, Irf4, Nrp1, Foxp3, and Bcl10 g were measured by qRT-PCR in WT (from Bcl10^+/+Foxp3^cre mice) and KO (from Bcl10^fl/flFoxp3cre mice) Treg cells (CD4⁺YFP⁺ cells) (mean ± SEM, n = 3). Student’s t test was used as the statistical test (n.s. = no significance, *p < 0.05, **p < 0.01, and ***p < 0.005). All error bars represent SDs