Fig. 6.

The TAK1 inhibitor 5Z-7-oxozeaenol suppresses MSU crystal-induced paw inflammation in C57BL6 mice. a, MSU (0.5 mg)-induced paw inflammation was measured over 48 h, and the efficacy of 5Z-7-oxozeaenol (5 or 15 mg/kg, p.o.) was evaluated. The change in paw circumference (Δ circumference) was determined and is presented as the mean ± SEM from n = 6 mice per group. b A histological analysis of H&E-stained slides was performed by a pathologist blinded to the study to obtain inflammation scores. c Representative pictures of treated and untreated ankles (upper panel) and H&E-stained sections (lower panel) from each group are shown. d Joint homogenates prepared from naive, MSU-, and MSU plus 5Z-7-ox (15 mg/kg; p.o.)-treated mice were subjected to Western blot analysis for p-TAK1 (Thr^184/187), total TAK1, p-IRAK4 (Thr^345/346), p-IRAK1 (Thr²⁰⁹), total IRAK1, TRAF6, and β-actin. e Western blot analysis of joint homogenates was performed to determine the expression levels of K⁴⁸-linked and K⁶³-linked ubiquitinated proteins. **p < 0.01 or ***p < 0.001 for naive vs. MSU alone or MSU vs. treatment; ^#p < 0.05 or ^##p < 0.01 for MSU v.s 5Z-7-oxozeaenol-treated