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. 2020 Jan 3;18(1):171–181. doi: 10.1038/s41423-019-0353-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Immunofluorescence staining shows E-cadherin expression on TECs cocultured with CD147 + CD4 + or CD147-CD4 + SP cells without TGFβ. b Western blot analysis results show E-cadherin expression in TECs cocultured with CD147 + CD4 + or CD147-CD4 + SP cells without TGFβ. c Immunofluorescence staining shows E-cadherin expression on TECs cocultured with CD147 + CD4 + or CD147-CD4 + SP cells in the presence of TGFβ. d Western blot analysis results show E-cadherin, Smad2 and p-Smad2 expression in TECs cocultured with CD147 + CD4 + or CD147-CD4 + SP cells in the presence of TGFβ. e Real-time PCR was performed to analyze the expression of FoxC2 in TECs cocultured with CD147 + CD4 + or CD147-CD4 + SP cells in the presence of TGFβ. f Immunofluorescence staining shows E-cadherin expression on TECs cocultured with CD147 + CD4 + cells in the presence of TGFβ or a TGFβ inhibitor. g Western blotting shows E-cadherin, Smad2 and p-Smad2 expression in TECs cocultured with CD147 + CD4 + cells in the presence of TGFβ or a TGFβ inhibitor. These images were obtained through the oil immersion lens of a confocal microscope (1000×), and the data are representative of three experiments.