Fig. 5. EMT of TECs can be induced by the interaction between Annexin A2 on TECs and CD147 on T cells in the presence of TGFβ.

a IF staining analysis of E-cadherin expression on TECs cocultured with CD147 + CD4 + cells in a contact or noncontact system in the presence of TGFβ (600×). b Western blot analysis results of E-cadherin expression on TECs cocultured with CD147 + CD4 + cells in a contact or noncontact system in the presence of TGFβ. c Mass spectrometry analysis results of the co-IP-obtained proteins. d PSMs and Log₁₀ (score) analysis results of the co-IP-obtained proteins. e The locations of Annexin A2 and E-cadherin in TECs, as determined using confocal microscopy (600×). f Western blot analysis results of E-cadherin expression in TECs cocultured with CD147 + CD4 + or CD147-CD4 + cells in the presence of TGFβ and anti-Annexin A2. g Western blot analysis results of p-E-cadherin and p-Src expression in TECs cocultured with CD147 + CD4 + or CD147-CD4 + cells in the presence of TGFβ. h Western blot analysis results of E-cadherin expression in TECs cocultured with CD147 + CD4 + or CD147-CD4 + cells in the presence of TGFβ and a Src inhibitor. The data in c and d are representative of two experiments, and the data in the other panels are representative of three experiments.