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. 2020 Sep 7;27(12):3226–3242. doi: 10.1038/s41418-020-00615-9

Fig. 2. PRMT1 elevates the expression of EZH2 protein.

Fig. 2

a–d Protein and mRNA expression of EZH2 were assessed by western blots (a, c) and qRT-PCR assays (b, d) after overexpression PRMT1. e–h EZH2 proteins and mRNAs were detected by western blot (e, g) and qRT-PCR (f, h) after knockdown PRMT1. i, j EZH2 protein and mRNA levels were analyzed by immunoblotting and qRT-PCR experiments in MM-231 cells treated by PRMT1 inhibitor AMI-1 (8.8uM). k–n Detection of EZH2 protein expression in MM-231, MCF7, BT549 and 4T1 cells treated by AMI-1 using different time-point. o EZH2 and PRMT1 expression levels in MCF10A cells and a variety of breast cancer cell lines. p, q IF analysis of PRMT1 and EZH2 in MM-231 cells treated by DMSO and AMI-1 or MM-231-shCtrl cells and MM-231-shPRMT1#1 cells, respectively. Data are represented as mean ± SEM of three independent experiments, and ns means no significance (Student’s t test).