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. 2020 Sep 7;27(12):3226–3242. doi: 10.1038/s41418-020-00615-9

Fig. 5. PRMT1-mediated meR342-EZH2 promotes its ability in silencing target gene transcriptional expression.

Fig. 5

a, b A series of EZH2 target genes mRNA expression levels were analyzed by qRT-PCR assays in MM-231-shCtrl and MM-231-shPRMT1#1 cells (a) and MM-231 cells treated by DMSO or AMI-1 (b), respectively. c EZH2 target genes mRNA expression were examined by qRT-PCR assays in MM-231-shEZH2 cells overexpressing Vector, Flag-EZH2-WT and Flag-EZH2-R342K, respectively. d–i ChIP assays were performed using anti-EZH2 (d, f and h) and anti-H3K27me3 (e, g and i) antibodies, and the immunoprecipitated DNA was analyzed by qRT-PCR using specific primers of HOXA10, DAB2IP, HOXA9 and HOXA7 promoters. Data are represented as mean ± SEM of three independent experiments, and ns means no significance, *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).