Figure 1.

Interfacial activity of rhSP-D. (A) Recording of surface pressure as a function of time for the adsorption of an aliquot of 10 μL at 0.34 mg/mL (3.4 μg) of rhSP-D injected into the subphase of a Wilhelmy balance. (B) Adsorption and spreading isotherms of rhSP-D upon injection of an aliquot of 20 μL at 0.6 mg/mL (12 μg) of the protein at the air-liquid interface in a double-Wilhelmy balance. Surface pressure measured in the donor (black line) and recipient troughs (gray line). (C) Fluorescence emission spectra of the F-rhSP-D detected in the whole volume taken from the recipient trough at the end of the experiments. Data represented by the mean and standard deviations of three different replicates.