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rhSP-D association to the interface in the absence of surfactant-associated reservoirs. (A) Spreading isotherm of OE in organic solvent in a double-Langmuir balance and (B) AFM height image obtained from the transference of the recipient interfacial film after the spreading of 360 μg of OE in organic solvent and the injection of 5% w/w rhSP-D 10 min after (black arrow in A). Three replicates were performed to obtain mean and standard deviation data.
