Skip to main content
. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Cell Stem Cell. 2020 Apr 16;26(6):880–895.e6. doi: 10.1016/j.stem.2020.03.013

Figure 2. Dermal adipocytes undergo lipolysis after injury.

Figure 2.

(A) Representative PLIN1 immunostained images of DWAT at the periphery of wounds and quantification of adipocyte cross sectional area and numbers (n ≥ 6 mice each time point). Scale bars, 100μm. (B) Representative immunostained images and quantification of CD68+ cells at the periphery of wound beds and in non-wounded (NW) skin (n ≥ 6 mice each time point). Scale bars, 100μm. (C) Transmission electron microscopy of adipocytes in uninjured skin and adipocytes at the wound periphery 1 day after injury. Asterisks show small lipid droplets. Quantification of adipocytes containing small lipid droplets at the periphery of a larger lipid droplet (n = 4 mice each condition). Scale bars, 5μm. (D) Experimental approach for quantitative lipidomics. (E–F) Lipid mass spectrometry quantification of non-esterified free fatty acid (FFA) classes (E) (n = 4–5 mice each condition) and FFA species (F) (n = 4–5 mice each time point). L, lipid droplet; SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid. Error bars indicate mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001.

See also Figure S3.