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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Cell Stem Cell. 2020 Apr 16;26(6):880–895.e6. doi: 10.1016/j.stem.2020.03.013

Figure 4. Adipocyte-derived cells migrate into wound beds.

Figure 4.

(A–F) Schematic of tamoxifen labeling strategy and immunostained images of GFP+ cells in day 7 wounds. Lower magnification images show dermal adipocytes (GFP+) and wound beds. Higher magnification panels are representative images from inside wound beds. Red box in A is representative of the wound bed location displayed in low magnification images in B–F. Scale bars, 500μm in lower magnification panels and 100μm in higher magnification panels. (G) Images of GFP+ cells in the center of wound beds from Rosa26CreER; mT/mG mice treated with tamoxifen at different stages of wound healing. (H) Schematic of adipocyte lineage tracing and PLIN1 immunostained sections in non-wounded (NW) skin and inside 7-day wound beds (WB). Scale bars, 100μm. (I) Labeling scheme to identify AdipoqCreER-traced cells. (J) Images of GFP+ cells in wound beds 8 weeks after injury. (K) LipidTOX staining at the wound edge near a growing peripheral hair follicle (hf). Scale bars, 50μm. White dotted lines delineate wound edges.

See also Figure S5.