Table 1. Cut-off, Sensitivity, Specificity and Accuracy of antibody detection to the 17 different arbovirus recombinant proteins on the reference panel of 161 human samples.
| Antigens | Cut-off calculation method | Cut-off value (MFIa) | N tested/ N negative | Specificity (%) | 95% CIb | N tested/ N negative | Sensitivity (%) | 95% CI | Accuracy (%) | 95% CI |
|---|---|---|---|---|---|---|---|---|---|---|
| CHIKV_E2 | Mean+3SD | 324 | 66/65 | 98.48 | 91.0–99.0 | 27/24 | 88.89 | 71.0–96.0 | 96 | 92.0–97.0 |
| ROC | 229 | 66/63 | 95.45 | 87.0–98.0 | 27/26 | 96.3 | 40.0–75.0 | 96 | 92.0–97.0 | |
| CHIKV_NSP | Mean+3SD | 395 | 66/65 | 98.48 | 0.91–99.0 | 27/12 | 44.44 | 27.0–62.0 | 83 | 75.0–84.0 |
| ROC | 246 | 66/65 | 98.48 | 0.91–99.0 | 27/20 | 74.07 | 55.0–86.0 | 91 | 86.0–93.0 | |
| ONNV_E2 | Mean+3SD | 362 | 66/64 | 96.97 | 0.91–99.0 | NA/NA | NA | NA | NA | NA |
| ZIKV_DIII | Mean+3SD | 485 | 66/65 | 98.48 | 0.91–99.0 | 16/0 | 0 | 0.0–19.0 | 79 | 70.0–80.0 |
| ROC | 145 | 66/59 | 89.39 | 79.0–94.0 | 16/02 | 12.5 | 03.0–36.0 | 74 | 65.0–75.0 | |
| ZIKV_NS1 | Mean+3SD | 74 | 66/65 | 98.48 | 91.0–99.0 | 16/16 | 100 | 80.0–100 | 0.99 | 96.0–100 |
| ROC | 345 | 66/66 | 100 | 94.0–100 | 16/16 | 100 | 80.0–100 | 100 | 100–100 | |
| YFV_NS1 | Mean+3SD | 421 | 66/65 | 98.48 | 91.0–99.0 | 18/5 | 27.78 | 12.0–50.0 | 83 | 75.0–85.0 |
| ROC | 176 | 66/62 | 93.94 | 85.0–97.0 | 18/8 | 44.44 | 24.0–66.0 | 83 | 75.0–85.0 | |
| DENV1_DIII | Mean+3SD | 134 | 66/66 | 100 | 94.0–100 | 23/17 | 73.91 | 53.0–87.0 | 93 | 88.0–95.0 |
| ROC | 132 | 66/66 | 100 | 94.0–100 | 23/18 | 78.26 | 58.0–91.0 | 94 | 90.0–96.0 | |
| DENV2_DIII | Mean+3SD | 842 | 66/65 | 98.48 | 91.0–99.0 | 23/4 | 17.39 | 06.0–37.0 | 78 | 69.0–78.0 |
| ROC | 631 | 66/63 | 95.45 | 87.0–98.0 | 23/8 | 34.78 | 18.0–55.0 | 80 | 71.0–81.0 | |
| DENV3_DIII | Mean+3SD | 197 | 66/63 | 95.45 | 87.0–98.0 | 23/17 | 73.91 | 53.0–87.0 | 90 | 84.0–91.0 |
| ROC | 79 | 66/57 | 86.36 | 76.0.-92.0 | 23/21 | 91.3 | 76.0–97.0 | 88 | 81.0–89.0 | |
| DENV4_DIII | Mean+3SD | 544 | 66/64 | 96.97 | 91.0–99.0 | 23/6 | 26.09 | 12.0–46.0 | 79 | 70.0–80.0 |
| ROC | 112 | 66/53 | 80.3 | 69.0–88.0 | 23/18 | 78.26 | 58.0–91.0 | 80 | 71.0–81.0 | |
| DENV1_NS1 | Mean+3SD | 673 | 66/65 | 98.48 | 91.0–99.0 | 23/21 | 91.3 | 73.0–97.0 | 97 | 93.0–98.0 |
| ROC | 550 | 66/64 | 96.97 | 91.0–99.0 | 23/22 | 95.65 | 79.0–99.0 | 97 | 93.0–98.0 | |
| DENV2_NS1 | Mean+3SD | 812 | 66/64 | 96.97 | 91.0–99.0 | 23/23 | 100 | 85.0–100 | 98 | 95.0–99.0 |
| ROC | 996 | 66/66 | 100 | 94.0–100 | 23/23 | 100 | 85.0–100 | 100 | 100–100 | |
| DENV3_NS1 | Mean+3SD | 54 | 66/64 | 96.97 | 91.0–99.0 | 23/23 | 100 | 85.0–100 | 98 | 95.0–99.0 |
| ROC | 200 | 66/66 | 100 | 94.0–100 | 23/23 | 100 | 85.0–100 | 100 | 100–100 | |
| DENV4_NS1 | Mean+3SD | 471 | 66/65 | 98.48 | 91.0–99.0 | 23/19 | 82.61 | 62.0–93.0 | 94 | 90.0–96.0 |
| ROC | 462 | 66/65 | 98.48 | 91.0–99.0 | 23/20 | 86.96 | 67.0–95.0 | 96 | 91.0–97.0 | |
| USUV_NS1 | Mean+3SD | 89 | 66/65 | 98.48 | 91.0–99.0 | NA/NA | NA | NA | NA | NA |
| WNV_NS1 | Mean+3SD | 100 | 66/64 | 96.97 | 91.0–99.0 | 11/11 | 100 | 74.0–100 | 97 | 94.0–99.0 |
| ROC | 1297 | 66/66 | 100 | 94.0–100 | 11/11 | 100 | 74.0–100 | 100 | 100–100 | |
| WNV_DIII | Mean+3SD | 309 | 66/65 | 98.48 | 91.0–99.0 | 11/11 | 100 | 74.0–100 | 99 | 96.0–100 |
| ROC | 817 | 66/66 | 100 | 94.0–100 | 11/11 | 100 | 74.0–100 | 100 | 100–100 |
(CHIKV: Chikungunya virus); (ZIKV: Zika virus); (DENV: Dengue virus); (USUV: Usutu virus); (WNV: West Nile virus); (YFV: Yellow Fever virus); (ONNV: O’Nyong Nyong virus).
a MFI: median fluorescence intensity
b CI: confidence interval.