Fig 6. FESEM (at 30,000X magnification) and fluorescence microscopy (at 1000X magnification) of biofilm formed by PAW1.

FESEM of untreated biofilm with extracellular matrix (a) and reduced numbers of acetic acid treated cells with disrupted extracellular matrix (b). Fluorescence images of the same samples at 528 nm (green) for SYTO9 signal and 645 nm (red) for PI signal were merged. Viable cells (green) are more in untreated sample (c) as compared to acetic acid treated sample (d) which also shows yellow red fluorescence of dead cells with a disrupted matrix. Bar lines in (a) and (b) indicate 3 μm while in (c) and (d) indicate 5 μm.