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. 2021 Feb 2;19(2):e3001044. doi: 10.1371/journal.pbio.3001044

Fig 1. Generation of MIR172 mutants by CRISPR-Cas9 technology.

Fig 1

(A) Location of the MIR172 genes on Arabidopsis chromosomes. The short and long arms of chromosomes 2, 3, and 5 are shown. (B) The genomic structure of the MIR172 gene loci in WT and the mir172 mutants. Orange, stem-loop of MIR172 gene; green, miRNA*; blue, miRNA. (C) Validation of the mir172 mutants. The WT or mutated versions of MIR172 genes were cloned and overexpressed in WT. Please note that overexpression of the WT version of MIR172 led to early flowering time phenotype. Scale bars represent 1 cm. (D) Flowering time measurement. The number of rosette leaves was counted. Plants were grown at 22°C in long days. The statistically significant differences are determined by ordinary one-way ANOVA (p < 0.05). See also S4 Table. The data underlying this figure are included in S1 Data. CRISPR, clustered regularly interspaced short palindromic repeats; miRNA, microRNA; sgRNA, single guide RNA; WT, wild type.