Fig 4. Expression pattern of MIR172 genes in long days.

(A–D) Analyses of MIR172 reporters. Plants were grown at 22°C in long days. The leaves (upper panels) and shoot apices (lower panels) were examined. Please note that the plants start to flower 3 weeks (approximately 21 days) after seed germination. Over 20 T₁ independent lines for each reporter were examined, and the results of 1 representative T₂ line are shown. Dash line marks the SAM. Scale bars represent 100 μm. (E–I) Expression of MIR172 genes (E–H) and AP1 (I) in WT. AP1 was monitored as an indication for floral transition. Plants were grown at 22°C in long days and harvested at different time points. Expression was normalized to TUB. Two technical replicates for each biological replicate (n = 2) were performed. Error bars represent SD. (J) Expression of the MIR172B reporter in WT and the spl9 mutant. One representative leaf of the same developmental age is shown. Scale bars represent 100 μm. The same confocal settings were used for scanning for each reporter line (A–D and I). The data underlying this figure are included in S1 Data. AP1, APETALA1; SAM, shoot apical meristem; TUB, β-TUBULIN-2; WT, wild type.