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. 2021 Feb;27(2):638–641. doi: 10.3201/eid2702.203824

Table. Results of PCR for severe acute respiratory syndrome coronavirus 2 in a pregnant woman and fetus, Brazil, 2020*.

Sample Day Cycle threshold†
ORF1ab N RNaseP‡
Maternal nasopharyngeal swab sample 0 21.0 24.0 23.0
Maternal nasopharyngeal swab sample 4 20.9 24.8 29.9
Umbilical cord blood 8 31.9 30.3 27.0
Placenta§ 8 24.5 25.5 25.6
Fetal liver 9 Undetectable Undetectable 29.0
Fetal spleen 9 Undetectable Undetectable 27.8
Fetal lungs 9 Undetectable Undetectable 25.7
Fetal central nervous system 9 Undetectable Undetectable 29.4
Fetal skeletal muscle 9 Undetectable Undetectable 26.5
Fetal heart 9 Undetectable Undetectable 26.5
Fetal ovary 9 Undetectable Undetectable 25.4

*PCR conducted using XGEN MASTER COVID-19 Kit (Mobius Life Science, Inc, https://mobiuslife.com.br). N, nucleocapsid protein gene; ORF, open reading frame.
†Cycle threshold value is considered positive if both viral genes are <38.
‡PCR is selective for human RNaseP gene as a control for sample integrity.
§Insufficient sample.