Figure 2.

RNA sequencing of human brain endothelial (HBE) cell media and plasma from children recruited in 2006, Mozambique. A) Percentage of mapped reads in different species of small RNAs, for both in vitro and ex vivo approaches. B) Ten most expressed miRNAs in HBE cell medias and plasmas. Color-coded cells show the percentage of each assay/condition (columns) for each miRNA (rows). C) Volcano plot of differentially expressed miRNAs in cell-condition media of niEs versus cell-condition media of iEs with the FCR3-ePCR strain (ePCR-iE) incubated with HBE cells. D) Volcano plot of differentially expressed miRNAs in cell-condition media of iEss with 3D7 strain (3D7-iE) versus cell-condition media of iEs with the FCR3-ePCR strain (ePCR-iE) incubated with HBE cells. Comparisons depicted in C and D were adjusted for multiple testing by the Benjamini-Hochberg method. Negative log₂-fold change indicates overexpression in ePCR-iE samples. ePCR, endothelial protein-C receptor (a binding Plasmodium falciparum strain); HRP2, histidine-rich protein 2; iE, infected erythrocyte; miRNA, microRNA; SM, severe malaria; UM, uncomplicated malaria.