Fig. 8.

Expression profiles of RGT genes in ovules and other tissues. (A) Expression of RGT complex-encoding genes in WT ovules during megasporogenesis and development of 2–4 nucleate female gametophytes (FG2-4). Nucellar tissue and FG2-4 samples were laser microdissected and analyzed by hybridization to Affymetrix arrays as described in Tucker et al. (2012a). The rest of the ovule tissues from dissected samples were collected separately. Mean normalized gene expression values for the Col WT nucellus, female gametophyte, and whole ovule ±SD are presented. (B) RNASEQ reads from the experiment SRP075604 were downloaded from publicly available databases (Klepikova et al., 2015, 2016). Reads were mapped to the Arabidopsis genome, and gene expression was calculated and presented as a heat map for selected plant organs.