Fig. 4. Modulation of octanol avoidance by Providencia requires the OCTR-1 OA receptor in the ASH sensory neurons.
a-b, Reversal response latency of animals of the indicated worm genotypes grown on the indicated bacteria on NGM + 0.5% L-Tyr (a) and supplemented with the indicated concentrations of TA (b) to 100% octanol using SOS assays. Dots, response time of single worms. Y-axis is log10-scaled and normalized to the indicated control group (a) or to the wild-type control (b) for each experimental day. Numbers in parentheses, number of worms tested in assays over at least 3 independent days. Wild-type octr-1 sequences were expressed in ASH and ASI under the srv-11 and srg-47 promoters, respectively. Boxplot, median and quartiles, whiskers, data range (excluding outliers). Gray thin and thick vertical bars, Bayesian 95% and 66% credible intervals for the difference of means. P-values between indicated conditions are from a LMM with Tukey-type multivariate-t adjustment. P-values in red, Wald t-statistic representing the genotype x food interaction effect relative to WT. c, Cartoon depicting short-range bacterial choice assay. d-e, Relative preference index of wild-type or mutant animals grown on the indicated bacteria for the test bacteria JUb39. Data are normalized to the OP50 control condition for each genotype. Dots, single assays of at least 10 animals. Numbers in parentheses, number of assays over at least 5 independent days. Y-axis is on log-odds ratio (logit) scale. Errors are SEM. Gray thin and thick vertical bars, Bayesian 95% and 66% credible intervals, respectively. P-values represent difference of means relative to JUb39-grown animals from a GLMM with Dunnett-type multivariate-t adjustment. P-value in red, Wald z-statistic representing the genotype x food interaction effect relative to WT (e). Allele used: tbh-1(n3247) (e). f, Cartoon of working model.