Extended Data Fig. 3. Complementation of TA-containing metabolites in tdc-1 mutants via Providencia TDC-encoding genes.

a, Overview of MS2 network obtained in positive ion mode. Each node represents a unique feature, and edges between nodes indicate similarity between MS2 spectra. Red circle highlights the sub-network containing the majority (16 out of 24 features, representing 10 out of 15 differential compounds) of differential TA-containing metabolites. This sub-network contains glycosylated TA-derivatives, N-succinyl-TA is represented by a node in a different sub-network (red arrow). b, Magnified view of sub-network highlighted in (a). The TA-containing features restored upon growth of tdc-1 worms on wild-type JUb39, and abolished when grown on JUb39 ΔtyrDC::cmR ΔadcA, are highlighted in light red. MS2 spectra for two example compounds at m/z 499.1474 and m/z 604.1689 (circled in magenta) are shown in Extended Data Figs. 4 and 5.