Extended Data Fig. 5. MS2 analysis and quantitation of TA-containing metabolite m/z 604.1689.
a, Major fragmentation reactions of m/z 604.1689 and resulting fragment ions. Stereochemistry and exact substitution pattern are unknown. The shown structure is based on fragmentation and stable isotope incorporation. b-c, MS2 spectra obtained in (b) negative- and (c) positive-ion mode. Representative data are shown from at least three biologically independent experiments. d, Quantification of m/z 604.1689 in wild-type and tdc-1 worms fed the indicated bacterial strains as determined by positive-ion ESI HPLC-MS. Dots, independent samples from n=3 experiments. Errors are SEM.