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. 2021 Feb 2;20:33. doi: 10.1186/s12934-021-01528-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Schematic representation of the expression and display delivery systems. a Schematic representation of the domains of the constructs expressing recombinant versions of the eight proteins in the four different genetically engineered systems. b The panel illustrates schematically the display on the OM of each construct including 1) the full-length lipoprotein with its own signal peptide including lipobox lacking a carrier protein system and the mature portion of the lipoprotein fused to 2) AIDA-I, 3) Lpp’OmpA and 4) INP, InaK (N-domain + C-domain). In both panels (a and b), each protein domain is displayed with different colours. SP signal peptide, LP lipoprotein. OM outer membrane