Fig. 5.

Expression and surface display of InaK fusion proteins in E. coli, at 18 °C. Western blot of whole-cell lysates showing InaK fusions proteins. Lane 1: Marker; Lane 2: T7ExpressIq expressing InaK-NmBamE,at 18 °C (a). FACS analysis of E. coli T7ExpressIq (pET15b) expressing InaK fused the N. meningitidis lipoprotein BamE at 18 °C (b) was incubated with polyclonal anti-NmBamE antibodies. The grey area represents the fluorescence signal obtained with the control (T7ExpressIq pET15b ∅). The light blue coloured line represents the fused form of the NmBamE lipoprotein