Table 1. Primers used to prepare segment-specific fluorescent probes.

(from Sicard et al., 2019 )

Name	Primer sequence	Size	Segment
NV C-297 F	ATGGGTCTGAAATATTTCTC	510nt	C
NV C-806 R	TTAATTAATTACAATCTCC
M ORF For2	GCTGCGTATCAAGACGAC	268nt	M
M ORF Rev2	TTCTAGCATCCCAATTCC
N ORF For	TGGCAGATTGGTTTTCTAGT	450nt	N
N ORF Rev	TTCTGAGTGAATGTACAATAAACATTT
R ORF For	ACATTAAATAATCCTCTCTCTCCTA	810nt	R
R ORF Rev	CCTATCATCACTAAACATGCC
S ORF For	AAATGGTGAGCAATTGGAA	350nt	S
S ORF Rev	GCCTATGATAGTAATCATATCTTGACA
U1 ORF For	TTGGTCGATTATTTGTTGGTT	455nt	U1
U1 ORF Rev	AATATCTCATTAGCATTAATTACATTTGAA
U2 ORF For	TTATGGATGCCGGCTTT	361nt	U2
U2 ORF Rev	CATGAAGTATTAGAATAACGAACTTGA
U4 ORF For	AGCAGGTTATGGAATGTAGG	313nt	U4
U4 ORF Rev	ATAGATTCCCACAATCGCT
R1 For	ACATTAAATAATCCTCTCTCTCCTA	282nt	R
R1 Rev	CTCTAAGCGTACTTGCAG
R2 For	GAAGAAAGCTATTGCTACTTGG	406nt
R2 Rev	CCTATCATCACTAAACATGCC
Nmys2-Red	TTACATCATAAGTGCTGTAAGTATAATAAGGCACAACTAA	40 nt	N
Nmys7-Green	TTACATCATATTATACCAACTGTTGCTATCCCACAACTAA	40 nt	N
Smys1-Red	GTTTTTCATTAACTTCTCACTATAATAACTGCCCACAGTG	40 nt	S
Smys8-Green	GTTTTTCATTAATATAAGTCATAATTCCTGCTCCACAGTG	40 nt	S