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| Primer | Sequence (5′-3′) |
|---|---|
| AceIN-F3 Mu1-G F | CTTTGGAAAGGACCAGGAAAGCTCCTC |
| AceIN-F3 Mu1-G R | CCTGGTCCTTTCCAAAGTGGATTTCTG |
| AceIN-F3 Mu2-A F | CTTTGGAAAGGACCAGAAAAGCTCCTC |
| AceIN-F3 Mu2-A R | TCTGGTCCTTTCCAAAGTGGATTTCTG |
Note: Introduced mutant is shown in Red font. F: forward primer; R: reverse primer; Mu: mutant.
