Table 3. PCR reaction mixes of the construction of two HIV-1 mutants.

Component	Volume (μl)
pUC57-IN (1-10 ng)	2
Mu F (10 μM)	1
Mu R (10 μM)	1
2×TransStart FastPfu PCR Supermix	25
Nuclease-free water	21
total	50

Note: F: forward primer; R: reverse primer; Mu: mutant.