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. 2019 Dec 5;9(23):e3447. doi: 10.21769/BioProtoc.3447

Figure 5. Waveform analyses of tone pip–evoked ABRs.

Figure 5.

[These data were described in Ingham et al. (2017) ]. Black lines and symbols indicate data recorded from 12 wildtype littermate control mice ( EphA2+/+). Red lines and symbols indicate data recorded from 23 EphA2 homozygous mutant mice (EphA2-/-). A and B. ABRs recorded from individual mice evoked by 40 dB SL 18 kHz tone pips. C. Mean amplitudes of the wildtype and mutant responses shown in A and B are plotted. Positive-deflection wave components are indicated by P1-P4. Negative-deflection wave components are indicated by N1-N4. D. Input/Output Functions for the peak-peak amplitude of waves 1-4 are plotted. Mean (± standard deviation) amplitude is plotted against sound level above threshold (dB SL, sensation level). E. Input/Output Functions for the peak latency of waves P1-P4 and N-N4 are plotted. Mean (± standard deviation) latency is plotted against sound level above threshold (dB SL). F. Input/Output Functions for 3 inter-peak intervals are plotted. Mean (± standard deviation) interval time is plotted against sound level above threshold (dB SL). These data illustrate that ABR responses between individual animals can be highly reproducible. In this case, there were no significant differences in the waveform response properties recorded from littermate wildtype and EphA2 mutant mice.