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. 2019 Oct 20;9(20):e3399. doi: 10.21769/BioProtoc.3399

Figure 1. Grinding procedure.

Figure 1.

. A. Cool down the Polyvials, steel grinding adapter, and steel caps in liquid nitrogen. B. Remove the plastic lid of the Polyvials and place the steel cap frozen. C. Use a capper/decapper machine (custom-made) to close the vial with the steel cap. D. Closed vial. E. Place the Polyvial inside steel grinding adapter. F. Place the grinding adapter in the Retsch mill and proceed as in Step A3. After grinding use the same capper/decapper machine for replacing the steel caps for the plastic lids. The samples can be stored at -80 °C or proceed to Step A4. Note that to avoid overheating of the vial, steel caps and grinding adapter deep them in liquid nitrogen or make use of dried ice while preparing a second sample.