Figure 4. Representative image to distinguish SIRF signals at various stages of the cell cycle.

The above panel shows an example of RAD51 SIRF (red channel) in HAP-1 cells treated with 125 μM EdU for 8 min and 200 μM HU for 4 h. The arrows indicate cells in late S-phase based on EdU patterning. Note that the green signal for Alexa 488 azide co-click enables distinction of late S-phase versus early S-phase cells. scale bars = 20 μm.