Table 1. Purification guidelines for Chlamydia trachomatis serovars .
Serovar | MOI | DEAE pretreatment | Cycloheximide | Growth until harvest |
---|---|---|---|---|
A | 1 | Yes | Yes | 70-72 h |
B | 0.5 | Yes | Yes | 70-72 h |
B Jali 20 | 1 | Yes | Yes | 70-72 h |
B Har36 | 1 | Yes | Yes | 70-72 h |
Ba | 1 | Yes | Yes | 70-72 h |
C | 1 | Yes | Yes | 70-72 h |
D | 0.5 | Yes | Yes | 62-66 h |
E | 1 | Yes | Yes | 46-48 h |
F | 1 | Yes | Yes | 46-48 h |
G | 1 | Yes | Yes | 70-72 h |
H | 1 | Yes | Yes | 70-72 h |
I | 1 | Yes | Yes | 70-72 h |
J | 1 | Yes | Yes | 70-72 h |
K | 1 | Yes | Yes | 70-72 h |
L1 | 1 | No | Improves titer | 46-48 h |
L2 | 3-5 | No | Improves titer | 46-48 h |
L3 | 1 | No | Improves titer | 46-48 h |