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. 2019 Sep 5;9(17):e3347. doi: 10.21769/BioProtoc.3347

Figure 9. Example of IgG anti- (BSA)/plastic, -MDA-BSA, and -rabbit IgG ELISA data.

Figure 9.

. The figure shows ELISA plate setup for screening mAbs binding to BSA/plastic, MDA-BSA or rabbit IgG. In this example, a positive systemic lupus erythematosus (SLE) patient serum pool is used as a positive control reference for IgG anti-MDA reactivity (here dilution 1:100 is set as 100 Reactivity Units [RU]/ml) and a positive RF IgG containing RA patient serum pool is used as a positive control reference sample on the rabbit IgG surface (here dilution 1:500 is set as 100 RU/ml). Qualification of the mAb reactivity for comparison between ELISA runs can be made by fitting a linear or logarithmic line to the standard curves and extrapolating a relative reactivity in RU/ml from the absorbance values.