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. 2019 Sep 5;9(17):e3347. doi: 10.21769/BioProtoc.3347

Table 1. Reagent preparation for cells sorting.

Buffer Reagent Concentration 1x 96x + 10%
Sorting buffer Nuclease-free water - 8.00 840.00
PBS [10x] 0.50 52.50
DTT [100 mM] 1.00 105.00
RNAsin [40 U/μl] 0.50 52.50
total 10.00 1050.00