Table 1. Reagent preparation for cells sorting.
Buffer | Reagent | Concentration | 1x | 96x + 10% |
---|---|---|---|---|
Sorting buffer | Nuclease-free water | - | 8.00 | 840.00 |
PBS | [10x] | 0.50 | 52.50 | |
DTT | [100 mM] | 1.00 | 105.00 | |
RNAsin | [40 U/μl] | 0.50 | 52.50 | |
total | 10.00 | 1050.00 |