Table 5. Digestion mix 2.
| Reagent | Concentration | 1x |
|---|---|---|
| Nuclease-free water | - | 4.00 |
| NEB3 Buffer | [10x] | 5.00 |
| BSA | [20 μg/μl] | 0.50 |
| Enzyme (BsiWI) | [20 U/μl] | 0.50 |
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