| Cycle step | Temp | Time | Cycles |
|---|---|---|---|
| Initial Denaturation | 98 °C | 30 s | 1 |
| Denaturation | 98 °C | 10 s | 14 cycles |
| Annealing/Extension | 65 °C | 75 s | |
| Final Extension | 65 °C | 5 min | 1 |
| Hold | 4 °C |
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