Figure 1. Preparation of the leaves of 4-week-old Arabidopsis plants for particle bombardment.

A. Arabidopsis plants after growing under the 16 h/8 h light/dark cycle for 4 weeks. scale bar = 1 cm. B. Rosette leaves that had not yet bolted were cut from Arabidopsis plants shown in (A) by ophthalmic scissors and placed concentrically with abaxial side facing upward on the surface of solid Murashige & Skoog medium. scale bar = 1 cm.