4% (resolving gel)	14% (resolving gel)	4% (stacking gel)
Glycerol	0	3.2 g	0 g
Water	6.5 ml	6.1 ml	7.4 ml
Buffer	1.4 ml	2.8 ml	1.7 ml
AB	0.68 ml	4.8 ml	0.8 ml
10% DM	43 μl	86 μl	50 μl
TEMED	3.5 μl	7.0 μl	10 μl
10% APS	19 μl	38 μl	80 μl
Volume	8.5 ml	17 ml	10 ml