Table 5. Preparation of qPCR mix for library amplification.
| Reagent | Mix per sample (200 μl) |
|---|---|
| ddH2O | 60 μl |
| KAPA HiFi Uracil+ 2x mastermix | 100 μl |
| Indexing primer | 6 μl |
| NEB Universal primer | 6 μl |
| Bisulfite-converted DNA | 24 μl |
| 10x SYBR Green | 4 μl |
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