Table 6. qPCR amplification protocol.

Step	Time	Temp (°C)
1–Denaturation	45 s	98
2.1–Denaturation	15 s	98
2.2–Annealing	30 s	57
2.3–Extension	45 s	72
Stop during the exponential phase (after about 12-15 cycles)
3–Extend	7 min	72