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A. Eluted chromatography spectrum. Red curve: UV absorbance at 280 nm; Blue curve: Conductivity. B. SDS-PAGE. Lane Medi: Pre-loading sample (medium); FL: flow through; E(NR): non-reducing elutant; E(R): reduced elutant. The star (★) indicates the non-reducing Fab; the diamond () indicates the educed fragments of LC() and HC() ( Luo et al., 2019b ).
