Table 2. PCR reaction recipes for amplifying STII-VL-CL-phoA-STII-VH-CH1 fragments.
| Reagents | Volume |
| DNA template 50 ng/μl | 1 μl |
| PrimeSTAR Max (2x) | 25 μl |
| forward and reverse primers (10 μM) | 1 μl each |
| ddH2O | add to 50 μl |
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