Table 3. Double enzyme digestion reaction recipes.
| Reagents | volume |
| pPhoA plasmid (or Fab PCR fragments) | 1 μg |
| 10x CutSmart® Buffer | 5 μl |
| Bgl II and Nde I (or Bsa I) | 1 μl each |
| ddH2O | add to 50 μl |
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