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PCR result using primers FWD_Tn5/7_gt and REV_Tn5/7_gt for the amplification of Tn7-delivered insert (MRE-Tn7 insert), and PCR result using primers Tn7_gt1, Tn7_gt2, and Tn7_gt3 for amplification of a fragment of plasmid pMRE-Tn7 backbone (MRE-Tn7 backbone). M: Molecular marker. Sample 1: P. eucalypti 299R::MRE-Tn7-145; Lane 2: E. coli (pMRE-Tn7-145); Lane 3: pMRE-Tn7-145; C-: water control.
