Table 3. Primers.
Name | Target | Sequence 5' to 3' * | tm (°C)# |
FWD Tn5/7_gt | Tn5 and Tn7 mutant genotyping | ATGGTGAGCAAGGGCGAG | 58 |
REV_Tn5/7_gt | Tn5 and Tn7 mutant genotyping | CAACAGGAGTCCAAGCTCAG | 56 |
FWD_Tn5_gt | Tn5 mutant genotyping | CTGAGTAGGACAAATCCGCCG | 58 |
REV_Tn5_gt | Tn5 mutant genotyping | GCCTCGGCAGAAACGTTGG | 60 |
Tn7_gt1 | Tn7 mutant genotyping (backbone) | GAATTACAACAGTACTGCGATGAG | 55 |
Tn7_gt2 | Tn7 mutant genotyping (backbone) | GATCAACTCTATTTCTCGCGGG | 56 |
Tn7_gt3 | Tn7 mutant genotyping (backbone) | TACATAACGGACTAAGAAAAACACTACAC | 64 |
FWD_uidA | uidA gene | AACAGGTGGTTGCAACTGGA | 60 |
REV_uidA | uidA gene | TTGCTGAGTTTCCCCGTTGA | 60 |