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. 2019 Apr 5;9(7):e3199. doi: 10.21769/BioProtoc.3199

Table 3. Primers.

Name Target Sequence 5' to 3' * tm (°C)#
FWD Tn5/7_gt Tn5 and Tn7 mutant genotyping ATGGTGAGCAAGGGCGAG 58
REV_Tn5/7_gt Tn5 and Tn7 mutant genotyping CAACAGGAGTCCAAGCTCAG 56
FWD_Tn5_gt Tn5 mutant genotyping CTGAGTAGGACAAATCCGCCG 58
REV_Tn5_gt Tn5 mutant genotyping GCCTCGGCAGAAACGTTGG 60
Tn7_gt1 Tn7 mutant genotyping (backbone) GAATTACAACAGTACTGCGATGAG 55
Tn7_gt2 Tn7 mutant genotyping (backbone) GATCAACTCTATTTCTCGCGGG 56
Tn7_gt3 Tn7 mutant genotyping (backbone) TACATAACGGACTAAGAAAAACACTACAC 64
FWD_uidA uidA gene AACAGGTGGTTGCAACTGGA 60
REV_uidA uidA gene TTGCTGAGTTTCCCCGTTGA 60